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EXPARNA_P(1) User Commands EXPARNA_P(1) NAME exparna_p - manual page for exparna_p (LocARNA 2.0.0) DESCRIPTION exparna_p: fast exact structure local matching of RNAs. USAGE: exparna_p [options] <Input 1> <Input 2> exparna_p performs pairwise matching and folding, i.e. it determines exactly matching local favourable sub-structures that can be simulata- neously formed in the input RNAs. Input Input consists of two sequences or alignments, which are specified in fasta, clustal, stockholm, or LocARNA pp format. Optionally, one can specify structure and anchor constraints in these input files. Output The program enumerates exactly matching local substructures (exact pat- tern matches = EPMs) and optionally chains them. It returns lists of chained and unchained matches, visualizations of the results and anchor constraints for alignment. OPTIONS -h, --help Help -V, --version Version info -v, --verbose Verbose -q, --quiet Quiet Scoring parameters: --no-stacking Do not use stacking terms (otherwise needs stacking probs by RNAfold -p2) --alpha_1=<factor>(1) Multiplier for sequential score --alpha_2=<factor>(5) Multiplier for structural score --alpha_3=<factor>(5) Multiplier for stacking score, 0 means no stacking contribution --struct-mismatch-score=<score>(-10) Score for a structural mismatch (nucleotide mismatch in an arc- match) Heuristics for speed accuracy trade off: -p, --min-prob=<prob>(0.01) Minimal probability --max-bps-length-ratio=<factor>(0.0) Maximal ratio of #base pairs divided by sequence length (de- fault: no effect) --max-uil-length-ratio=<factor>(0.0) Maximal ratio of #unpaired bases in loops divided by sequence length (default: no effect) --max-bpil-length-ratio=<factor>(0.0) Maximal ratio of #base pairs in loops divided by loop length (default: no effect) -D, --max-diff-am=<diff>(30) Maximal difference for sizes of matched arcs -d, --max-diff=<diff>(-1) Maximal difference for alignment traces --max-diff-at-am=<diff>(-1) Maximal difference for alignment traces, only at arc match posi- tions --prob_unpaired_in_loop_threshold=<prob>(0.01) Threshold for prob_unpaired_in_\ loop --prob_basepair_in_loop_threshold=<prob>(0.01) Threshold for prob_basepair_in_\ loop Output: -p, --out-min-prob=<prob>(0.0005) Minimal probability for output (min-prob overrides if smaller) --output-ps Output best EPM chain as colored postscript -a, --PS_fileA=<file>() Postscript output file for sequence A -b, --PS_fileB=<file>() Postscript output file for sequence B -o, --output-locarna=<file>() Fasta file with anchor constraints for locarna --output-anchor-pp=<fileroot>() PP files <fileroot>_A.pp and <fileroot>_B.pp, merging input PPs and anchor constraints from chaining --output-clustal=<file>() Write file with chain as alignment in clustalw format --output-epm-list=<file>() A list of all found epms --output-chained-epm-list=<file>() A list of all EPMs that are present in the chain --inexact-struct-match Allow inexact structure matches --add-filter Apply an additional filter to enumerate only EPMs that are maxi- mally extended (only inexact) --no-chaining Do not use the chaining algorithm to find best overall chain Suboptimal traceback: --subopt Use the suboptimal traceback --diff-to-opt-score=<threshold>(-1) Threshold for suboptimal traceback --min-score=<score>(90) Minimal score of a traced EPM --number-of-EPMs=<threshold>(100) Maximal number of EPMs for the suboptimal traceback Constraints: --noLP use --noLP option for folding --maxBPspan=<span>(-1) Limit maximum base pair span (default=off) --relaxed-anchors Relax anchor constraints (default=off) Miscellaneous: --stopwatch Print run time information. Input files: The two input files <Input 1> and <Input 2> specify the input sequences in various, automatically detected formats. Accepted formats are: Fasta, Clustal, Stockholm LocARNA PP, ViennaRNA postscript dotplot. Unless in-loop probabilities are provided (only possible in LocARNA PP), base pair probabilities are computed by partition function folding. Clustal, Stockholm, and PP input can contain constraints. AVAILABILITY The latest LocARNA package release is available online at at Github https://github.com/s-will/LocARNA and http://www.bioinf.uni- freiburg.de/Software/LocARNA/ REFERENCES Christina Otto, Mathias Mohl, Steffen Heyne, Mika Amit, Gad M. Landau, Rolf Backofen, and Sebastian Will. ExpaRNA-P: simultaneous exact pat- tern matching and folding of RNAs. BMC Bioinformatics, 15:404, 2014. doi:10.1186/s12859-014-0404-0 EXAMPLES Essentially exparna_p is called in the same way as locarna and the other pairwise alignemnt tools of the package. Please refer to their documentation. Note that exparna is usually called with single se- quences rather than alignments. AUTHOR This man page is written and maintained by Sebastian Will it is part of the LocARNA package. The exparna_p tool and the library classes for strong ensemble-based sparsification were written by Christina Otto. REPORTING BUGS Report bugs to <schmiedc (at) informatik.uni-freiburg.de>. COPYRIGHT Copyright 2005- Christina Otto, Sebastian Will. The LocARNA package is released under GNU Public License v3.0 SEE ALSO The LocARNA PP 2.0 format is described online at http://www.bioinf.uni- freiburg.de/Software/LocARNA/PP/ exparna_p (LocARNA 2.0.0) November 2022 EXPARNA_P(1)
NAME | DESCRIPTION | OPTIONS | AVAILABILITY | REFERENCES | EXAMPLES | AUTHOR | REPORTING BUGS | COPYRIGHT | SEE ALSO
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