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samtools-targetcut(1)	      Bioinformatics tools	   samtools-targetcut(1)

NAME
     samtools targetcut - cut fosmid regions (for fosmid pool only)

SYNOPSIS
     samtools  targetcut [-Q minBaseQ] [-i inPenalty] [-0 em0] [-1 em1] [-2 em2]
     [-f ref] in.bam

DESCRIPTION
     This command identifies target regions by examining the continuity of  read
     depth,  computes  haploid	consensus sequences of targets and outputs a SAM
     with each sequence corresponding to a target. When option -f is in use, BAQ
     will be applied. This command is only designed for  cutting  fosmid  clones
     from fosmid pool sequencing [Ref. Kitzman et al. (2010)].

OPTIONS
     -Q minBaseQ
	     Ignore bases with quality less than minBaseQ.

     -i inPenalty
	     Penalty for in state transition.

     -0 em0  Emission score 0.

     -1 em1  Emission score 1.

     -2 em2  Emission score 2.

     -f ref  Reference FASTA file.

AUTHOR
     Written by Heng Li from the Sanger Institute.

SEE ALSO
     samtools(1)

     Samtools website: <http://www.htslib.org/>

samtools-1.23.1 		  18 March 2026 	   samtools-targetcut(1)

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