VCFALL...ITIVES(1) vcfallelicprimitives (VCF transformation) VCFALL...ITIVES(1) NAME vcfallelicprimitives - Converts stdin or given VCF file and reduces alle- les. SYNOPSIS vcfallelicprimitives DESCRIPTION vcfallelicprimitives converts stdin or given VCF file to tab-delimited for- mat, Realign reference and alternate alleles with WFA, parsing out the primitive alleles into multiple VCF records. New records have IDs that reference the source record ID. Genotypes are handled. Deletion alleles will result in haploid (missing allele) genotypes. Note that this tool is considered legacy and will emit a warning! Use vcfwave instead. Options -h, -help shows help message and exits. See more below. EXIT VALUES 0 Success not 0 Failure EXAMPLES >>> head("vcfallelicprimitives -h",29) > usage: ./vcfallelicprimitives [options] [file] > WARNING: this tool is considered legacy and is only retained for older workflows. It will emit a warning! Even though it can use the WFA you should use [vcfwave](./vcfwave.md) instead. > Realign reference and alternate alleles with SW or WF, parsing out the primitive alleles into multiple VCF records. New records have IDs that reference the source record ID. Genotypes are handled. Deletion alleles will result in haploid (missing allele) genotypes. > options: -a, --algorithm TYPE Choose algorithm SW (Smith-Waterman) or WF wavefront (default: WF) -m, --use-mnps Retain MNPs as separate events (default: false). -t, --tag-parsed FLAG Annotate decomposed records with the source record position (default: ORIGIN). -L, --max-length LEN Do not manipulate records in which either the ALT or REF is longer than LEN (default: unlimited). -k, --keep-info Maintain site and allele-level annotations when decomposing. Note that in many cases, such as multisample VCFs, these won't be valid post decomposition. For biallelic loci in single-sample VCFs, they should be used with caution. -d, --debug debug mode. > Type: transformation vcfallelicprimitives picks complex regions and simplifies nested align- ments. For example: >>> sh("grep 10158243 ../samples/10158243.vcf") grch38#chr4 10158243 >3655>3662 ACCCCCACCCCCACC ACC,AC,ACCCCCACCCCCAC,ACCCCCACC,ACA 60 . AC=64,3,2,3,1;AF=0.719101,0.0337079,0.0224719,0.0337079,0.011236;AN=89;AT=>3655>3656>3657>3658>3659>3660>3662,>3655>3656>3660>3662,>3655>3660>3662,>3655>3656>3657>3658>3660>3662,>3655>3656>3657>3660>3662,>3655>3656>3661>3662;NS=45;LV=0 GT 0|0 1|1 1|1 1|0 5|1 0|4 0|1 0|1 1|1 1|1 1|1 1|1 1|1 1|1 1|1 4|3 1|1 1|1 1|1 1|0 1|0 1|0 1|0 1|1 1|1 1|4 1|1 1|1 3|0 1|0 1|1 0|1 1|1 1|1 2|1 1|2 1|1 1|1 0|1 1|1 1|1 1|0 1|2 1|1 0 After aligning it reduces into two records with variant alleles 10158243:ACCCCCA/A 10158243:ACCCCCACCCC/A 10158243:ACCCCCACCCCCA/A 10158243:ACCCCCACCCCCAC/A 10158255:AC/A 10158255:ACC/A and adjusts the genotypes accordingly splitting into two records using the original (but arguably OBSOLETE) SW algorithm: >>> sh("../build/vcfallelicprimitives -a SW -m -L 1000 ../samples/10158243.vcf|grep -v ^\#") grch38#chr4 10158243 >3655>3662_1 ACCCCCACCCCCAC ACCCCCAC,ACAC,AC,A 60 . AC=3,1,64,3;AF=0.0337079,0.011236,0.719101,0.0337079;LEN=6,10,12,13;ORIGIN=grch38#chr4:10158243,grch38#chr4:10158243,grch38#chr4:10158243,grch38#chr4:10158243;TYPE=del,del,del,del GT 0|0 3|3 3|3 3|0 2|3 0|1 0|3 0|3 3|3 3|3 3|3 3|3 3|3 3|3 3|3 1|0 3|3 3|3 3|3 3|0 3|0 3|0 3|0 3|3 3|3 3|1 3|3 3|3 0|0 3|0 3|3 0|3 3|3 3|3 4|3 3|4 3|3 3|3 0|3 3|3 3|3 3|0 3|4 3|3 0 grch38#chr4 10158255 >3655>3662_2 ACC AC,A 60 . AC=2,1;AF=0.0224719,0.011236;LEN=1,2;ORIGIN=grch38#chr4:10158243,grch38#chr4:10158243;TYPE=del,del GT 0|0 .|. .|. .|0 2|. 0|0 0|. 0|. .|. .|. .|. .|. .|. .|. .|. 0|1 .|. .|. .|. .|0 .|0 .|0 .|0 .|. .|. .|0 .|. .|. 1|0 .|0 .|. 0|. .|. .|. .|. .|. .|. .|. 0|. .|. .|. .|0 .|. .|. 0 With the default wavefront algorithm we get a different result 1$10158244:CCCCCACCCCCAC/C 2$10158244:CCCCCACCCCCACC/C 3$10158245:CCCCACCCCCACC/C 4$10158251:CCCCACC/C 5$10158256:CC/C >>> sh("../build/vcfallelicprimitives -m -L 1000 ../samples/10158243.vcf|grep -v ^\#") grch38#chr4 10158244 >3655>3662_1 CCCCCACCCCCACC CC,C 60 . AC=1,3;AF=0.011236,0.0337079;LEN=12,13;ORIGIN=grch38#chr4:10158243,grch38#chr4:10158243;TYPE=del,del GT 0|0 0|0 0|0 0|0 1|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 2|0 0|2 0|0 0|0 0|0 0|0 0|0 0|0 0|2 0|0 0 grch38#chr4 10158245 >3655>3662_2 CCCCACCCCCACC C 60 . AC=64;AF=0.719101;LEN=12;ORIGIN=grch38#chr4:10158243;TYPE=del GT 0|0 1|1 1|1 1|0 .|1 0|0 0|1 0|1 1|1 1|1 1|1 1|1 1|1 1|1 1|1 0|0 1|1 1|1 1|1 1|0 1|0 1|0 1|0 1|1 1|1 1|0 1|1 1|1 0|0 1|0 1|1 0|1 1|1 1|1 .|1 1|. 1|1 1|1 0|1 1|1 1|1 1|0 1|. 1|1 0 grch38#chr4 10158251 >3655>3662_3 CCCCACC C 60 . AC=3;AF=0.0337079;LEN=6;ORIGIN=grch38#chr4:10158243;TYPE=del GT 0|0 .|. .|. .|0 .|. 0|1 0|. 0|. .|. .|. .|. .|. .|. .|. .|. 1|0 .|. .|. .|. .|0 .|0 .|0 .|0 .|. .|. .|1 .|. .|. 0|0 .|0 .|. 0|. .|. .|. .|. .|. .|. .|. 0|. .|. .|. .|0 .|. .|. 0 grch38#chr4 10158256 >3655>3662_4 CC C 60 . AC=2;AF=0.0224719;LEN=1;ORIGIN=grch38#chr4:10158243;TYPE=del GT 0|0 .|. .|. .|0 .|. 0|. 0|. 0|. .|. .|. .|. .|. .|. .|. .|. .|1 .|. .|. .|. .|0 .|0 .|0 .|0 .|. .|. .|. .|. .|. 1|0 .|0 .|. 0|. .|. .|. .|. .|. .|. .|. 0|. .|. .|. .|0 .|. .|. 0 grch38#chr4 10158257 >3655>3662_5 C A 60 . AC=1;AF=0.011236;LEN=1;ORIGIN=grch38#chr4:10158243;TYPE=snp GT 0|0 .|. .|. .|0 .|. 0|. 0|. 0|. .|. .|. .|. .|. .|. .|. .|. .|. .|. .|. .|. .|0 .|0 .|0 .|0 .|. .|. .|. .|. .|. .|0 .|0 .|. 0|. .|. .|. .|. .|. .|. .|. 0|. .|. .|. .|0 .|. .|. 0 ./vcfallelicprimitives -a SW -m -L 1000 ../sam- ples/grch38#chr8_36353854-36453166.vcf > ../test/data/regression/vcfall- elicprimitives_5.vcf run_stdout("vcfallelicprimitives -a SW -m -L 1000 ../samples/grch38#chr8_36353854-36453166.vcf", ext="vcf") output in vcfallelicprimitives_5.vcf ./vcfallelicprimitives -a SW -m -L 1000 ../sam- ples/grch38#chr4_10083863-10181258.vcf > ../test/data/regression/vcfall- elicprimitives_6.vcf /regression/vcfallelicprimitives_6.vcf >>> run_stdout("vcfallelicprimitives -a SW -m -L 1000 ../samples/grch38#chr4_10083863-10181258.vcf", ext="vcf") output in vcfallelicprimitives_6.vcf ./vcfallelicprimitives -L 10000 -m ../samples/grch38#chr8_36353854-36453166.vcf > ../test/data/regression/vcfallelicprimitives_7.vcf run_stdout("vcfallelicprimitives -L 10000 -m ../sam- ples/grch38#chr8_36353854-36453166.vcf", ext="vcf") output in vcfallelicprimitives_7.vcf ./vcfallelicprimitives -m ../samples/grch38#chr4_10083863-10181258.vcf > ../test/data/regression/vcfallelicprimitives_8.vcf run_stdout("vcfallelicprimitives -m ../sam- ples/grch38#chr4_10083863-10181258.vcf", ext="vcf") output in vcfallelicprimitives_8.vcf Another diff example where the first is SW and the second WFA2 showing: ```python >>> sh("diff data/regression/vcfallelicprimitives_6.vcf data/regression/vcfallelicprimitives_8.vcf|tail -6") 1670c1680,1682 < grch38#chr4 10180508 >4593>4597_1 CTT CTTT,CT,C 60 . AC=7,47,1;AF=0.0786517,0.52809,0.011236;LEN=1,1,2;ORIGIN=grch38#chr4:10180508,grch38#chr4:10180508,grch38#chr4:10180508;TYPE=ins,del,del GT 2|0 0|2 2|2 2|0 2|2 0|0 0|2 0|2 2|2 2|2 2|0 2|0 0|2 2|0 2|2 2|0 2|2 2|2 2|2 2|0 0|1 0|0 2|1 2|2 0|2 2|2 2|0 0|2 0|3 2|1 0|2 0|0 2|0 1|2 2|2 0|1 2|2 0|0 0|0 1|0 0|1 2|0 0|0 2|2 2 --- > grch38#chr4 10180508 >4593>4597_1 CTT C 60 . AC=1;AF=0.011236;LEN=2;ORIGIN=grch38#chr4:10180508;TYPE=del GT 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|1 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0 > grch38#chr4 10180509 >4593>4597_2 TT T 60 . AC=47;AF=0.52809;LEN=1;ORIGIN=grch38#chr4:10180508;TYPE=del GT 1|0 0|1 1|1 1|0 1|1 0|0 0|1 0|1 1|1 1|1 1|0 1|0 0|1 1|0 1|1 1|0 1|1 1|1 1|1 1|0 0|0 0|0 1|0 1|1 0|1 1|1 1|0 0|1 0|. 1|0 0|1 0|0 1|0 0|1 1|1 0|0 1|1 0|0 0|0 0|0 0|0 1|0 0|0 1|1 1 > grch38#chr4 10180510 >4593>4597_3 T TT 60 . AC=7;AF=0.0786517;LEN=1;ORIGIN=grch38#chr4:10180508;TYPE=ins GT .|0 0|. .|. .|0 .|. 0|0 0|. 0|. .|. .|. .|0 .|0 0|. .|0 .|. .|0 .|. .|. .|. .|0 0|1 0|0 .|1 .|. 0|. .|. .|0 0|. 0|. .|1 0|. 0|0 .|0 1|. .|. 0|1 .|. 0|0 0|0 1|0 0|1 .|0 0|0 .|. . shows how WFA2 is doing a better job at taking things apart. Even so, this record is wrong. From grch38#chr4_10083863-10181258.vcf Differences between WFA and biWFA: wdiff vcfallelicprimitives_8.vcf vcfwave_5.vcf grch38#chr4 10134337 >2103>2106_1 [-TTTTG AGGCA-] {+TTTTGGTGTACTGCCT AGGCAGTACACCAAAA+} grch38#chr4 10134492 >2125>2211_3 [-TTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTG GTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTG-] {+TTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAG GTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAGCATCCCAAGTGATGTAG+} grch38#chr4 10134498 grch38#chr4 [-10134501 >2125>2211_5 AATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATT CATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATT-] {+10134500 >2125>2211_6 GAATCCCAATTGATGGAG G+} 60 . [-AC=1;AF=0.011236;INV=0,0;LEN=17;ORIGIN=grch38#chr4:10134484;TYPE=complex-] {+AC=1;AF=0.011236;INV=0;LEN=17;ORIGIN=grch38#chr4:10134484;TYPE=del+} GT 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|1 0|0 0|0 0|0 0 grch38#chr4 {+10134518 >2125>2211_7 AATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATTGATGGAGAATCCCAATT CATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATTGATGGAGCATCCCAATT 60 . AC=1;AF=0.011236;INV=0;LEN=112;ORIGIN=grch38#chr4:10134484;TYPE=mnp GT 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 0|0 0|0 0 Another problem case fixed with fb7365b07f832bcfcc6ddb693ddfc4a01a25cfbb grch38#chr8_36353854-36453166.vcf:grch38#chr8 36382847 >721>726 GT GC,AC SW vcfallelicprimitives_5.vcf:grch38#chr8 36382847 >721>726_1 GT AC vcfallelicprimitives_5.vcf:grch38#chr8 36382848 >721>726_2 T C WF vcfwave_4.vcf:grch38#chr8 36382847 >721>726_1 G A Let's look at some longer sequences: The original grch38#chr4_10083863-10181258.vcf:grch38#chr4 10134514 >2136>2148 GGAGAATCCCAATTGATGG GTAGCATCCCAAGTGATGT,GTAGAATCCCAATTGATGT,GGAGCATCCCAATTGATGG,GG 60 . AC=11,7,1,3;AF=0.125,0.0795455,0.0113636,0.0340909;AN=88;AT=>2136>2138>2139>2141>2142>2144>2145>2147>2148,>2136>2137>2139>2140>2142>2143>2145>2146>2148,>2136>2137>2139>2141>2142>2144>2145>2146>2148,>2136>2138>2139>2140>2142>2144>2145>2147>2148,>2136>2138>2148;NS=45;LV=1;PS=>2125>2211 GT 0|1 1|0 0|0 0|1 0|0 1|0 1|01|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 2|2 0|0 4|0 0|0 0|1 0|10|1 0|2 0|0 4|0 0|2 0|0 0|0 2|0 0|0 0|0 0|0 0|0 0|0 2|04|1 0|0 0|0 0|0 0|0 0|3 0|0 0|2 0|0 1 # translates to SW vcfallelicprimitives_6.vcf:grch38#chr4 10134514 >2136>2148_1 GGAGAATCCCAATTGATG G 60.AC=3;AF=0.0340909;LEN=17;ORIGIN=grch38#chr4:10134514;TYPE=del GT 0|0 0|0 0|0 0|0 0|00|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 0|0 0|0 1|0 0|00|0 0|0 0|0 0|0 0|0 1|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|00|0 0|0 1|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0 vcfallelicprimitives_6.vcf:grch38#chr4 10134515 >2136>2148_2 GAGAATCCCAATTGATGG TAGAATCCCAATTGATGT,TAGCATCCCAAGTGATGT 60 . AC=7,11;AF=0.0795455,0.125;LEN=18,18;ORIGIN=grch38#chr4:10134514,grch38#chr4:10134514;TYPE=mnp,mnp GT 0|2 2|0 0|0 0|2 0|0 2|0 2|0 2|00|0 0|0 0|0 0|0 0|0 0|. 0|0 1|1 0|0 .|0 0|0 0|2 0|2 0|20|1 0|0 .|0 0|1 0|0 0|0 1|0 0|0 0|0 0|0 0|0 0|0 1|0 .|20|0 0|0 0|0 0|0 0|0 0|0 0|1 0|0 2 vcfallelicprimitives_6.vcf:grch38#chr4 10134518 >2136>2148_3 AATCCCAATTGATGG CATCCCAATTGATGG 60.AC=1;AF=0.0113636;LEN=15;ORIGIN=grch38#chr4:10134514;TYPE=mnp GT 0|0 0|0 0|0 0|0 0|00|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 0|0 0|0 .|0 0|00|0 0|0 0|0 0|0 0|0 .|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|00|0 0|0 .|0 0|0 0|0 0|0 0|0 0|1 0|0 0|0 0|0 0 # and WFA vcfallelicprimitives_8.vcf:grch38#chr4 10134515 >2136>2148_1 GAGAATCCCAATTGATGG TAGCATCCCAAGTGATGG,TAGAATCCCAATTGATGG,G 60 . AC=11,7,3;AF=0.125,0.0795455,0.0340909;LEN=14,16,17;ORIGIN=grch38#chr4:10134514,grch38#chr4:10134514,grch38#chr4:10134514;TYPE=mnp,mnp,del GT 0|1 1|0 0|00|1 0|0 1|0 1|0 1|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 2|2 0|03|0 0|0 0|1 0|1 0|1 0|2 0|0 3|0 0|2 0|0 0|0 2|0 0|0 0|00|0 0|0 0|0 2|0 3|1 0|0 0|0 0|0 0|0 0|0 0|0 0|2 0|0 1 vcfallelicprimitives_8.vcf:grch38#chr4 10134518 >2136>2148_2 AATCCCAATTGATG CATCCCAATTGATG 60.AC=1;AF=0.0113636;LEN=14;ORIGIN=grch38#chr4:10134514;TYPE=mnp GT 0|0 0|0 0|0 0|0 0|00|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|. 0|0 0|0 0|0 .|0 0|00|0 0|0 0|0 0|0 0|0 .|0 0|0 0|0 0|0 0|0 0|0 0|0 0|0 0|00|0 0|0 .|0 0|0 0|0 0|0 0|0 0|1 0|0 0|0 0|0 0 To describe the bug we get for grch38#chr4 10134514 (or: original, sw:, wf:). It was fixed with commit fb7365b07f832bcfcc6ddb693ddfc4a01a25cfbb. ori: GGAGAATCCCAATTGATGG->GG sw: GGAGAATCCCAATTGATG ->G wf: GAGAATCCCAATTGATGG->G fix: GAGAATCCCAATTGATGG->G ori: GGAGAATCCCAATTGATGG->GTAGCATCCCAAGTGATGT sw: GAGAATCCCAATTGATGG-> TAGAATCCCAATTGATGT wf: GAGAATCCCAATTGATGG-> TAGAATCCCAATTGATGG <- fix: GAGAATCCCAATTGATGG-> TAGAATCCCAATTGATGT ori: GGAGAATCCCAATTGATGG ->GTAGAATCCCAATTGATGT sw: GAGAATCCCAATTGATGG -> TAGCATCCCAAGTGATGT wf: GAGAATCCCAATTGATGG -> TAGCATCCCAAGTGATGG <- fix: GAGAATCCCAATTGATGG-> TAGCATCCCAAGTGATGT GAGAATCCCAATTGATGG-> TAGAATCCCAATTGATGG ori: GGAGAATCCCAATTGATGG->GGAGCATCCCAATTGATGG sw: AATCCCAATTGATGG-> CATCCCAATTGATGG wf: AATCCCAATTGATG -> CATCCCAATTGATG fix: AATCCCAATTGATGG-> CATCCCAATTGATGG A C vcfwave issue Now where does the result TAGAATCCCAATTGATGG come from? Original input record (see samples/10134514.vcf) 10134514 GGAGAATCCCAATTGATGG GTAGCATCCCAAGTGATGT,GTAGAATCCCAATTGATGT,GGAGCATCCCAATTGATGG,GG WF CIGARs: 10134514:1M1X2M1X7M1X5M1X:GGAGAATCCCAATTGATGG,GTAGCATCCCAAGTGATGT 10134514:1M1X16M1X:GGAGAATCCCAATTGATGG,GTAGAATCCCAATTGATGT 10134514:4M1X14M:GGAGAATCCCAATTGATGG,GGAGCATCCCAATTGATGG 10134514:2M17D:GGAGAATCCCAATTGATGG,GG Decomposed alleles (return from parsedAlternates): GGAGAATCCCAATTGATGG 10134514 GGAGAATCCCAATTGATGG -> GGAGAATCCCAATTGATGG GGAGCATCCCAATTGATGG 10134514 GGAG -> GGAG GGAGCATCCCAATTGATGG 10134518 A -> C GGAGCATCCCAATTGATGG 10134519 ATCCCAATTGATGG -> ATCCCAATTGATGG GTAGAATCCCAATTGATGT 10134514 G -> G GTAGAATCCCAATTGATGT 10134515 G -> T GTAGAATCCCAATTGATGT 10134516 AGAATCCCAATTGATG -> AGAATCCCAATTGATG GTAGAATCCCAATTGATGT 10134532 G -> T GTAGCATCCCAAGTGATGT 10134514 G -> G GTAGCATCCCAAGTGATGT 10134515 G -> T GTAGCATCCCAAGTGATGT 10134516 AG -> AG GTAGCATCCCAAGTGATGT 10134518 A -> C GTAGCATCCCAAGTGATGT 10134519 ATCCCAA -> ATCCCAA GTAGCATCCCAAGTGATGT 10134526 T -> G GTAGCATCCCAAGTGATGT 10134527 TGATG -> TGATG GTAGCATCCCAAGTGATGT 10134532 G -> T Final result (see test/data/regression/vcfwave_5.vcf): 10134515 GAGAATCCCAATTGATGG TAGAATCCCAATTGATGG,G 10134518 A C 10134526 T G 10134532 G T Now where does TAGAATCCCAATTGATGG come from? Output produced by test/tests/realign.py LICENSE Copyright 2011-2024 (C) Erik Garrison, Pjotr Prins and vcflib contributors. MIT licensed. AUTHORS Erik Garrison, Pjotr Prins and other vcflib contributors. vcfallelicprimitives (vcflib) VCFALL...ITIVES(1)
NAME | SYNOPSIS | DESCRIPTION | EXIT VALUES | EXAMPLES | ./vcfallelicprimitives -a SW -m -L 1000 ../sam- | ./vcfallelicprimitives -a SW -m -L 1000 ../sam- | ./vcfallelicprimitives -L 10000 -m ../samples/grch38#chr8_36353854-36453166.vcf | ./vcfallelicprimitives -m ../samples/grch38#chr4_10083863-10181258.vcf > | vcfwave issue | LICENSE | AUTHORS
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